Objective To investigate the best operation opportunity of laparoscopic cholecysteetomy to treat acute lithic cholecystitis. Methods Retrospective analysis the clinical data of 106 cases of acute lithic cholecystitis treated by laparoscopic cholecystectomy. 56 cases whose time from onset to operation ≤72h were divided into observation group, other 50 cases whose time from onset to operation 〉72h were divided into control group. Observation indexes including average operation time, the rate of conversion to open surgery, complication rate, pain time, anal exsufflation time and average hospitalization time were compared between these two groups. Results The average operation time, average hospitalization time, anal exsufflation time and pain time of observation group were shorter than control group（P〈0. 05）. The rate of conversion to open surgery and complication rate of observation group were lower than control group（P〈 0.05）. Conclusion The laparoscopic choleeystectomy of acute lithic cholecystitis should be carried out early. The best operation time is within 72 hours. The laparoscopic cholecystectomy of acute lithie cholecystitis is safety that doctors master correct time and conversion to open surgery in time.