Objective To probe the effect of long chain non-coding RNA (LncRNA) EPB41L4A-AS1 regulating microRNA-30e-5p (miR-30e-5p) on ovarian cancer cell proliferation, migration, and invasion. Methods QRT-PCR was used to detect the levels of LncRNA EPB41L4A-AS1 and miR-30e-5p in ovarian cancer cell lines (SKOV3, A2780, OVCAR3, SW626, HO-89100) and tissues. The dual luciferase reporter gene assay was applied to detect the binding relationship between LncRNA EPB41L4A-AS1 and miR-30e-5p. OVCAR3 cells were assigned into Control group, pcDNA-NC group, pcDNA-EPB41L4A-AS1 group, pcDNA-EPB41L4A-AS1+miR-NC group, and pcDNA-EPB41L4A-AS1+miR-30e-5p-mimics group. Edu, scratch healing and Transwell experiments were applied to detect the proliferation, migration, and invasion ability of OVCAR3 cells. Immunoblotting experiment was applied to detect the expression of relevant proteins. The nude mouse transplant tumor experiment was applied to verify the effect of LncRNA EPB41L4A-AS1 on the growth of ovarian cancer. Results The expression level of LncRNA EPB41L4A-AS1 was decreased and the expression level of miR-30e-5p was increased in ovarian cancer tissues and ovarian cancer cell lines (P<0.05). StarBase database prediction showed that LncRNA EPB41L4A-AS1 had binding sites with miR-30e-5p. Compared with the pcDNA-NC group, the positive rate of Edu, number of cell migration, and number of invasive cells decreased in the pcDNA-EPB41L4A-AS1 group, the expression of miR-30e-5p, CDK1, MMP-2, MMP-9, N-Cadherin, and Vimentin decreased, while the expression levels of LncRNA EPB41L4A-AS1 and E-Cadherin increased (P<0.05). Compared with the pcDNA-EPB41L4A-AS1+miR-NC group, the positive rate of Edu, number of cell migration, and number of invasive cells increased in the pcDNA-EPB41L4A-AS1+miR-30e-5p-mimics group, the expression of miR-30e-5p, CDK1, MMP-2, MMP-9, N-Cadherin, and Vimentin increased, while the expression levels of E-Cadherin decreased (P<0.05). The tumor volume and weight of the pcDNA-EPB41L4A-AS1 〖JP〗group were smaller than those of the pcDNA-NC group, the expression of LncRNA EPB41L4A-AS1 was higher than that of the pcDNA-NC group, and the expression of miR-30e-5p was lower than that of the pcDNA-NC group (P<0.05). Conclusion Overexpression of LncRNA EPB41L4A-AS1 inhibits malignant biological behaviors of ovarian cancer cells, such as proliferation, migration, and invasion, by targeting miR-30e-5p