Objective To explore the HR-HPV infection in high-risk patients with cervical cancer in Nanjing and the analysis of influencing factors.Methods Clinicopathological data of 10，184 patients who underwent cervical cancer screening from 2021 to 2022 in the Department of Obstetrics and Gynaecology of Eastern Theatre General Hospital were retrospectively analyzed. The screening protocol was HPV high-risk typing combined with TCT testing, with either positive followed by colposcopy. The frequency of HR-HPV in patients with suspected cervical lesions was counted, and the positive rates of TCT testing and colposcopic pathological testing were calculated.Results The top 3 subtypes of HPV infection in Nanjing were 16, 52, and 58, and the multiple infection rate was 39.34%; age was positively correlated with the number of HR-HPV infections, the older the age, the higher the number of HPV infections (P<0.001); and the higher the number of HPV infections, the higher the risk level of TCT detection (P<0.001). The local screening method in Nanjing, which used HPV high-risk typing combined with TCT testing, and colposcopy for any positive result, effectively detected various cervical lesions. Among them, 877 cases were HR-HPV positive, with a positive rate of 91.35%. Among 960 patients with suspected cervical lesions, 307 cases were TCT positive, with a TCT detection positive rate of 31.98%. The pathological positive rates of patients with different numbers of HR-HPV infections were all around 30%, indicating that this screening method can better improve the detection rate of cervical lesions. Conclusion HR-HPV16 and HR-HPV52 are the main types of infections among women in Nanjing, with a high rate of multiple infections, and the number of HPV infections is positively correlated with age and TCT test results； the screening method adopted in Nanjing, which is HPV high-risk typing combined with TCT testing and colposcopy for