Objective To investigate the effects of vacuum-assisted closure technique (VAC) on dorsalis pedis artery blood flow, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (bFGF) levels in patients with diabetic foot Wagner grade 3 and 4. Methods A total of 86 patients with Wagner grade 3 and 4 diabetic foot in our hospital from May 2021 to May 2023 were selected and divided into randomized control groups. The control group (43 cases) received conventional Western medicine treatment, and the observation group (43 cases) received VAC technology on this basis. The clinical efficacy, blood flow of dorsal foot artery, serum factors related to wound repair, wound healing, pain degree and quality of life were compared between the two groups. Results The clinical effective rate of observation group was higher than that of control group (P<0.05). At 3 months after operation, the blood flow velocity, inner diameter, RI, PI, VEGF, EGF and bFGF of dorsal foot artery in observation group were higher than those in control group (P<0.05). After the procedure, three months later, the observation group's ulcer area and wound pH value were less than the control group's, and the wound's partial oxygen pressure was greater (P<0.05). Three months following the procedure, the observation group's GQOLI-74 score was greater than the control group's, and its VAS score was lower (P<0.05) than the control group's. Conclusion VAC technology has a significant effect in the treatment of Wagner grade 3 and 4 diabetic foot patients, which can effectively regulate the blood flow of dorsal foot artery and the level of serum factors related to wound repair, promote wound healing, reduce the pain degree of patients, and improve the quality of life, with good application effects