Objective To explore and analyze the clinical characteristics and related risk factors of norovirus infection in children with acute diarrhea.Methods A total of 125 hospitalized children with acute diarrhea in the hospital were selected from January 2023 to June 2024 as the research subjects. The clinical data of all patients were collected, and the fecal samples were collected to detect norovirus by double real-time fluorescence PCR detection kit(TaqMan probe method). The virus detection results and clinical data were analyzed, and Logistic regression model was used to analyze the related risk factors of norovirus infection in children with acute diarrhea. Results By comparing the detection status of norovirus in fecal samples of patients with acute diarrhea at different time points, it was found that the detection rate of norovirus in the first half of 2024 was significantly higher than that in the first half of 2023 (P<0.05). There was no significant difference in the positive rate of norovirus among acute diarrhea patients with different genders (P>0.05), but the positive rate of norovirus among children with acute diarrhea at different ages and in different seasons was statistically significant (χ2=12.762, 19.769, P<0.05). There were statistically significant differences in the positive detection of norovirus in patients with acute diarrhea with different clinical characteristics, fecal properties and detection levels of white blood cells and red blood cells in feces (χ2=13.482, 13.012, 11.859, 11.618, P<0.05). According to univariate Logistic regression analysis, season, clinical characteristics, fecal properties, routine white blood cell and red blood cell were the risk factors of norovirus infection in children with acute diarrhea (P<0.05). Multivariate Logistic regression analysis was performed on the indicators with differences in univariate analysis, and the results showed that season, clinical characteristics, and fecal properties were independent risk factors for norovirus infection in children with acute diarrhea (P<0.05).Conclusion The number of children with acute diarrhea caused by norovirus infection shows an increasing trend year by year, and norovirus infection is more common in younger children. Season, clinical characteristics and fecal properties are independent risk factors for norovirus infection in children with acute diarrhea, and can be used as clinical judgment indicators, which is of great significance for the treatment and prevention of norovirus infection in children with acute diarrhea