Objective Based on bioinformatics analysis, to explore the expression characteristics of tumor necrosis factor receptor related factor 7 (TRAF7) in ovarian cancer tissue, construct a molecular diagnostic model and analyze its clinical diagnostic value for prognosis in ovarian cancer. Methods The expression of TRAF7 was analyzed on the GEPIA online website in pan cancer tissues. Using the Kaplan-Meier Plotter online website to analyze the relationship between TRAF7 expression and survival of ovarian cancer patients. Furthermore, 92 ovarian cancer patients admitted to our hospital from April 2020 to June 2021 were selected as the study subjects, divided into a poor prognosis group (n=42) and a good prognosis group (n=50). Immunohistochemical detection of TRAF7 expression in ovarian cancer tissues and analysis of its relationship with clinical and pathological features of ovarian cancer. Pearson correlation analysis was used to evaluate the correlation between TRAF7 and human epididymal protein 4 (HE4) and carbohydrate antigen 125 (CA125). The value of evaluating TRAF7, HE4, and CA125 levels using receiver operating characteristic (ROC) curves in diagnosing poor prognosis of ovarian cancer. Results GEPIA online website showed that the expression level of TRAF7 in ovarian cancer tissue was higher than that of the normal control group (P＜0.05). As the expression level of TRAF7 increases, the survival rate of patients decreases (Logrank P=0.029). The immunohistochemical results showed that the expression level of TRAF7 in ovarian cancer tissue was higher than that in normal tissue adjacent to cancer. The difference was statistically significant among patients with different FIGO stages, pathological types, tumor differentiation, and distant metastasis (all P＜0.05). The expression of TRAF7 was positively correlated with the levels of HE4 and CA125 (r=0.459, r=0.358, both P＜0.001). The area under the joint detection curve of the three was 0.873 (95% CI: 0.787～0.933), with a sensitivity of 0.8810 and a specificity of 0.7600. Conclusion The combined detection of TRAF7, HE4 and CA125 significantly improves the diagnostic efficiency of prognosis in ovarian cancer, providing valuable reference and evaluation basis for screening high-risk populations with poor prognosis in ovarian cancer