Objective To explore the expression and mechanism of SKA1 in endometrial cancer. Methods Analyzed the effect of SKA1 on EC in GEPIA database. RT-qPCR was used to detect the expression levels of SKA1 in tumor tissues and normal tissues, as well as in human endometrial cancer cells and normal cells. The proliferation, colony formation, cell cycle distribution, migration, and invasion of RL95-2 and Ishiwaka cells were measured by CCK-8 assay, plate cell clone formation assay, flow cytometry, scratch assay, and Transwell method. Protein immunoblotting was used to detect the levels of SKA1, CyclinD1, MMP2, MMP9, GAPDH, P13K, p-P13K, AKT and p-AKT in two groups of cells. Results SKA1 was highly expressed in EC, and its high expression was associated with reduced survival rate in EC patients. SKA1 showed high expression in tumour tissues, RL95-2 and Ishiwaka cells. Si-SKA1 could inhibit the proliferation, migration and invasion ability of RL95-2 and Ishiwaka cells. SKA1 could downregulate the expression of SKA1, CyclinD1, MMP2 and MMP9 by inhibiting the PI3K/AKT pathway. Conclusion SKA1 can promote the proliferation, migration and invasion of endometrial cancer cells through the PI3K/AKT pathway