Lymphomas are highly heterogeneous, traditional bulk high-throughput sequencing only captures the average profile of abundant cells yet and fails to provide the extensive genetic information required for accurate diagnosis and therapy, posing a major challenge to precision oncology. Single-cell sequencing, however, enables analysis at the individual-cell level, retrieving low-abundance critical cellular information and dissecting the tumor micro-environment among malignant cells. Consequently, it facilitates lymphoma subtyping, precise diagnosis, therapeutic stratification, and investigation of drug-resistance mechanisms. Now this paper summarizes studies that have employed single-cell sequencing to dissect the lymphoma tumor micro-environment and discuss the breakthroughs and future prospects this technology brings to precision diagnosis and treatment of lymphoma