Objective To investigate the potential molecular mechanism of salvia miltiorrhiz-Rupaeony in the treatment of retinal vein occlusion (RVO) by using bioinformatics and network pharmacology. Methods The active components and target genes of salvia miltiorrhiza and Radix Paeoniae were screened by TCMSP and UniProt. The retinal vein blocking disease genes were then obtained in GeneCards and OMIM databases. The active drugs and target gene networks of salvia miltiorrhiza and Radix Paeoniae were constructed using CytoScape. The STRING online tool builds target protein interaction (PPI) network models. The Metascape bioinformatics analysis platform was used for Gene Ontology (GO) and Kyoto Genome Encyclopedia (KEGG) pathway enrichment analysis.Results In this study, 74 active natural products and 232 potential target genes from Salvia miltiorrhiza and Radix paeoniae were screened. PPI analysis identified 14 most potential salviorrhiza-Red peony target genes for RVO treatment, including JUN, AKT1, STAT3, TP53, MAPK1, TNF, etc. The results of bioinformatics analysis showed that the main life activities involved DNA binding transcription factor activity regulation, growth factor-related response, leukocyte growth and differentiation, etc. The main signaling pathways involved AGE-RAGE signaling pathway related to diabetes complications, fluid shear force and arteriosclerosis signaling pathway, MAPK signaling pathway, TNF signaling pathway, etc. 〖WTHZ〗Conclusion This analysis suggests that salvia miltiorrhiza and Red Peony may play multiple roles in the treatment of RVO, such as anti-arteriosclerosis, anti-inflammatory and antithrombotic