Objective To evaluate the diagnostic value of kisspeptin, asprosin and retinol-binding protein (RBP) in infertility in patients with polycystic ovary syndrome (PCOS). Methods 118 patients with PCOS admitted to our hospital from June 2021 to June 2023 were selected as PCOS group. 75 patients who underwent physical examination in our hospital during the same period were selected as control group. The serum levels of kisspeptin, asprosin and RBP were observed in the two groups. The univariate and multivariate analysis of infertility, and the diagnostic value of serum kisspeptin, asprosin in predicting infertility were also observed in PCOS patients. Results The serum levels of kisspeptin, asprosin and RBP in PCOS group were significantly higher than those in control group (P＜0.01), and the serum levels of kisspeptin, asprosin and RBP were increased with the increase of PCOS severity (P＜0.01). The incidence of infertility with induced abortion and intrauterine adhesion was significantly higher than that in the non-occurrence group (P＜0.01), and the levels of serum kisspeptin, asprosin and RBP in the occurrence group were significantly higher than those in the non-occurrence group (P＜0.01). Multifactor regression analysis showed that the increased levels of serum kisspeptin, asprosin and RBP in PCOS patients were independent risk factors for infertility (P＜0.05). The serum levels of kisspeptin, asprosin and RBP had high diagnostic value of infertility in PCOS patients. The sensitivity of combined detection was 96.2%, the specificity was 90.8%, and the AUC was 0.982, which were significantly higher than the kisspeptin (z=3.432, P=0.001) and asprosin (z=2.804, P=0.001). P=0.005) and RBP (z=3.925, P＜0.001), while there was no significant difference in AUC among them (P＞0.05). 〖WTHZ〗Conclusion The kisspeptin, asprosin and RBP are involved in the occurrence and development of PCOS disease, which are indicators of the severity of PCOS disease, and have good clinical value in predicting infertility in PCOS patients