Objective To analyze the correlation between pelvic organ prolapse and urodynamic and lower urinary tract symptoms in female patients with stress urinary incontinence, so as to provide a reference for clinical diagnosis and treatment. Methods A retrospective analysis was performed for 332 female inpatients with SUI admitted to The First Affiliated Hospital of Xinxiang Medical College from January 2017 to December 2023, including 204 patients with simple stress urinary incontinence and 128 patients with pressure urinary incontinence with pelvic organ prolapse. A total of 128 patients with pressure urinary incontinence with pelvic organ prolapse were divided into 29 patients with degree Ⅰ. 31 cases in degree Ⅱ，35 cases in degree Ⅲ, and 33 cases in degree Ⅳ group, according to the POP quantitation (POP-Q) of the anterior vaginal wall. Results There were significant differences in urodynamic parameters between the patients with simple SUI and the group with SUI (P<0.05). The results showed that the maximum urinary flow rate in the third-degree group and the fourth-degree group were smaller than those of the first-degree group, and the difference was statistically significant (P<0.05). The maximum urinary flow rate in the Ⅳ degree group was smaller than that in the Ⅱ degree group, and the difference was statistically significant (P<0.05). The mean uroflow rate of the Ⅳ degree group was significantly lower than that of the Ⅰ degree group and the the Ⅱ group(P<0.05). The urine flow of degree Ⅰ, degree Ⅱ, and degree Ⅲ group was significantly greater than that of degree Ⅳ group(P＜0.05). Different POP-Q stages of the anterior vaginal wall were negatively correlated with the maximum urinary flow rate (r=-0.293,P<0.01) and negatively correlated with the mean urinary flow rate (r=-0.258,P<0.01). It was negatively correlated with urine flow (r=-0.253,P<0.01). The proportions of patients with at least one lower urinary tract symptom in the Ⅰ, Ⅱ, Ⅲ, and Ⅳ groups were 44.8%, 58.1%, 71.4%, and 87.9%, respectively. Conclusion POP has an effect on UDS parameters in female patients with SUI. The degree of anterior vaginal wall prolapse is negatively correlated with the maximum urinary flow rate, mean urinary flow rate, and urine flow rate in female patients with SUI. Urodynamic examination has important clinical application value in female SUI patients with POP, which provides an important reference for the clinical diagnosis of SUI complicated with POP, and provides clinical guidance for individualized diagnosis and treatment. As the degree of prolapse increases, the proportion of patients with lower urinary tract symptoms may increase