Objective To investigate the role of JAK-STAT pathway and IL-23、MCP-1 and FN1 in HaCaT cells with UV irradiation. Methods HaCaT cells were irradiated with different dose of UV（30、60、90 mj/cm2）, after 24 h, microscope observed cell morphology. The enzyme linked immunosorbent assay(Elisa) to measure the levels of IL-23, FN1 and MCP-1 in the culture lysate of HaCaT cells; the levels of IL-23 mRNA, FN1 mRNA and MCP-1 mRNA was detected by qRT-PCR, the levels of TYK2, p-STAT4 and p-STAT6 were measured by Western-blot. Results Compared with the control group, the levels of IL-23, FN1 and MCP-1 increased, the levels of IL-23 mRNA, FN1 mRNA and MCP-1 mRNA were increased, the levels of TYK2, p-STAT4 and p-STAT6 were increased by the increase doses of UV. Conclusion JAK-STAT pathway and IL-23, FN1 and MCP-1 play a role in HaCaT with UV irradiation