Objective To investigate the expression of apoptosis suppressor gene survivin and matrix metalloproteinase-2 (MMP-2) protein in gastric cancer and their relationship with Helicobacter pylori (Hp) infection.Methods Ninety patients with gastric cancer who underwent radical gastrectomy in The Second People's Hospital of Wuxi from May 2020 to May 2022 were retrospectively selected as research objects. Clinical data of the selected patients were analyzed, and gastric cancer tissues and paracancer tissues were collected. The expression of survivin and MMP-2 in gastric and paracancer tissues and Hp infection, the positive expression of survivin and MMP-2 in gastric cancer tissues, and the clinicopathological features were compared. The correlation between the expressions of survivin and MMP-2 and Hp infection in gastric cancer patients was analyzed by Pearman method. Receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of survivin and MMP-2 in the diagnosis of Hp -positive gastric cancer.Results The survivin and MMP-2 positive expression rates in gastric cancer tissues were higher than those in paracancer tissues (P<0.05). The positive expression rates of Survivin and MMP-2 in Hp-positive gastric tissues were significantly higher than Hp-negative ones (P<0.05). In patients with gastric cancer, the positive expression rates of survivin and MMP-2 in Ⅲ~Ⅳ were significantly higher than those in Ⅰ~Ⅱ (P<0.05). In the degree of differentiation, survivin and MMP-2 positive expression rates of low-differentiation were significantly higher than those of medium-high differentiation (P<0.05). In the depth of infiltration, the survivin and MMP-2 positive expression rates of patients with serous membrane invasion were significantly higher than those without serous membrane invasion (P<0.05). The survivin and MMP-2 positive expression rates in patients with lymph node metastasis were significantly higher than those without lymph node metastasis (P<0.05). Pearman correlation analysis showed that survivin and MMP-2 expression in gastric cancer patients were positively correlated with Hp infection (r=0.427; 0.484, P<0.05). The AUCs of survivin and MMP-2 were 0.667, 0.639, and 0.879, respectively. The AUC of combined diagnosis was higher than that of each single test, and the sensitivity and specificity of combined detection were 84.40% and 78.90%.Conclusion Survivin and MMP-2 are highly expressed in gastric cancer patients, and they may be closely related to tumor stage, degree of differentiation, depth of invasion, and lymph node metastasis. Hp infection is positively correlated with Survivin and MMP-2 positive expression in gastric cancer. Combined detection of Survivin and MMP-2 expression is helpful to evaluate the disease of patients with gastric cancer and Hp positive