Objective To investigate the clinical significance of PDZK1IP1 and immune infiltration in gliomas. Methods The expression levels of PDZK1IP1 in gliomas from the TCGA database and brain tissues from the GTEx database were analyzed using R software. mRNA-seq and clinical data from the TCGA database were used to analyze the relationship between PDZK1IP1 expression and important clinical pathological features (age, isocitrate dehydrogenase (IDH) status, 1p/19q co-deletion, WHO grade) in gliomas. Kaplan-Meier analysis was performed to assess the relationship between PDZK1IP1 expression and overall survival (OS). GO and KEGG analyses were conducted to explore the biological functions and potential signaling pathways enriched by PDZK1IP1. Spearman correlation analysis was used to investigate the relationship between PDZK1IP1 expression and immune cell infiltration in tumor tissues. Results PDZK1IP1 was significantly enriched in gliomas, and its expression level was significantly associated with patient age, IDH status, 1p/19q co-deletion, and WHO grade. Patients with high PDZK1IP1 expression had worse OS PDZK1IP1 expression was significantly correlated with neutrophil and macrophage infiltration in the tumor microenvironment. Conclusion PDZK1IP1 may serve as a potential biomarker in gliomas, potentially promoting malignant progression by modulating the complex tumor immune microenvironment in gliomas