包裹间充质干细胞的大鲵皮肤粘液凝胶敷料制备研究
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军队医学科技青年培育计划课题资助项目(20QNPY156)


Study on the preparation of giant salamander skin mucus gel dressing wrapped with mesenchymal stem cells
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    摘要:

    目的 以大鲵皮肤粘液作为凝胶基质,同时将间充质干细胞和血管内皮生长因子装载于凝胶内部,研究新型的伤口敷料,观察其对创面的修复效果。方法 刮取大鲵皮肤粘液,采用真空冷冻干、低温超微粉碎等方法,将大鲵皮肤分泌物粉末制备成大鲵皮肤粘液凝胶,采用HE染色以及扫描电镜等方法观察大鲵皮肤粘液凝胶基质材料学表征,明确大鲵皮肤粘液凝胶的内部结构。之后,将骨髓间充质干细胞(BMSC)孵育于大鲵皮肤粘液凝胶基质,然后将血管内皮生长因子(VEGF)与其共培养,促使BMSC向内皮细胞分化。完成包裹间充质干细胞的大鲵皮肤粘液凝胶的材料学表征、表面生物相容性等方面评价。建立创伤动物模型,分别将凝胶敷料和生理盐水的医用纱布敷在伤口处,分别记为凝胶处理组和生理盐水组。观察伤后第4、14、20天两组伤口流血、红肿、渗出、瘙痒、结痂和脱痂状态、表面平整度与皮肤弹性等指标,并比较愈合率。结果 成功制备包裹间充质干细胞的大鲵皮肤粘液凝胶敷料,通过HE染色、扫描电镜、免疫荧光、罗丹明染色等可观察到凝胶敷料中包裹的间充质干细胞。通过创伤愈合相关实验结果可以观察到,在创伤后大鲵皮肤粘液凝胶敷料处理组的创面愈合情况较生理盐水组效果显著,愈合率明显高于生理盐水组。结论 包裹间充质干细胞的大鲵皮肤粘液凝胶敷料能促进创面的愈合

    Abstract:

    Objective To study a new wound dressing and observe its repairing effect on the wound surface by taking giant salamander skin mucus as the matrix of gel and loading mesenchymal stem cells and Vascular endothelial growth factor into the gel. Methods The skin mucus of giant salamander was scraped, and the powder of the skin secretion of giant salamander was prepared into the skin mucus gel of giant salamander by vacuum freeze-drying, low-temperature ultramicro pulverization and other methods. The material characterization of the skin mucus gel matrix of giant salamander was observed by HE staining and scanning electron microscopy, and the internal structure of the skin mucus gel of giant salamander was clarified. After that, bone marrow mesenchymal stem cells (BMSCs) were incubated in the giant salamander skin mucus gel matrix, and then Vascular endothelial growth factor (VEGF) was co cultured with BMSCs to promote the differentiation of BMSCs into endothelial cells. The evaluation of the material characterization and surface biocompatibility of the giant salamander skin mucus gel wrapped with mesenchymal stem cells was completed. Complete the wound healing function study, establish the wound animal model, apply gel dressing and normal saline medical gauze to the wounds of the treatment group and the control group respectively, observe the bleeding, redness, exudation, pruritus, scab formation and desquamation status, surface smoothness and skin elasticity of the wounds of the treatment group and the control group on the 4th, 14th and 20th days after injury, and compare the healing rate. Results The giant salamander skin mucus gel dressing wrapped with mesenchymal stem cells was successfully prepared. The mesenchymal stem cells wrapped in the gel dressing could be observed by HE staining, scanning electron microscopy, immunofluorescence, Rhodamine staining, etc. Through the relevant experimental results of wound healing, it could be observed that the wound healing of the treatment group of giant salamander skin mucus gel dressing after trauma was significantly better than the control group, and the healing rate was significantly higher than the control group. Conclusion The Andrias davidianus skin mucus gel dressing wrapped with mesenchymal stem cells can promote wound healing

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  • 在线发布日期: 2024-12-20
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