Abstract:Objective To study a new wound dressing and observe its repairing effect on the wound surface by taking giant salamander skin mucus as the matrix of gel and loading mesenchymal stem cells and Vascular endothelial growth factor into the gel. Methods The skin mucus of giant salamander was scraped, and the powder of the skin secretion of giant salamander was prepared into the skin mucus gel of giant salamander by vacuum freeze-drying, low-temperature ultramicro pulverization and other methods. The material characterization of the skin mucus gel matrix of giant salamander was observed by HE staining and scanning electron microscopy, and the internal structure of the skin mucus gel of giant salamander was clarified. After that, bone marrow mesenchymal stem cells (BMSCs) were incubated in the giant salamander skin mucus gel matrix, and then Vascular endothelial growth factor (VEGF) was co cultured with BMSCs to promote the differentiation of BMSCs into endothelial cells. The evaluation of the material characterization and surface biocompatibility of the giant salamander skin mucus gel wrapped with mesenchymal stem cells was completed. Complete the wound healing function study, establish the wound animal model, apply gel dressing and normal saline medical gauze to the wounds of the treatment group and the control group respectively, observe the bleeding, redness, exudation, pruritus, scab formation and desquamation status, surface smoothness and skin elasticity of the wounds of the treatment group and the control group on the 4th, 14th and 20th days after injury, and compare the healing rate. Results The giant salamander skin mucus gel dressing wrapped with mesenchymal stem cells was successfully prepared. The mesenchymal stem cells wrapped in the gel dressing could be observed by HE staining, scanning electron microscopy, immunofluorescence, Rhodamine staining, etc. Through the relevant experimental results of wound healing, it could be observed that the wound healing of the treatment group of giant salamander skin mucus gel dressing after trauma was significantly better than the control group, and the healing rate was significantly higher than the control group. Conclusion The Andrias davidianus skin mucus gel dressing wrapped with mesenchymal stem cells can promote wound healing