Objective To construct a dog's MODS model by injection of endotoxin leading to sepsis in dogs and the formation of multiple organ failure. By studying the indicators of kidneys, we can further observe the kidney damage of MODS dogs, and better understand the mechanism of kidney damage in MODS dogs, and provide theoretical basis for clinical and experimental research. Methods Fourteen dogs were divided into two groups, including 7 dogs with multiple organ failure models (experimental group) and 7 normal dogs (control group). Learn more about kidney damage by measuring kidney indicators over time. Results According to the experiment, the MODS model dog group (experimental group) compared with the normal dog group (control group), the experimental group canine indicators of renal function urea nitrogen and creatinine began to show a progressive increase 2 hours after the start of the experiment (P<0.05), the urine output of the experimental group was significantly reduced compared with the control group, and the time of polyuria was significantly delayed. The pathological results showed that sepsis would cause damage to the renal tubules, but diffuse necrosis did not occur (P<0.05).Conclusion In the experimental dog, the kidney indicators began to rise 2 hours after the model was successfully established. The kidney did have acute damage. Pathology also proved the kidney damage, but the renal tubules did not undergo diffuse necrosis. Experiments have confirmed that if you want to save the kidney function of experimental dogs, you need to actively search for the cause of kidney damage 2 hours ago, actively treat the primary disease, and provide theoretical support for the clinic. The kidney is one of the important organs of the body, and the pathogenesis of MODS can be better understood by studying the mechanism of its acute injury