Objective To explore the effect of suspension training combined with interference electrotherapy on rehabilitation of patients with non-specific low back pain. Methods A total of 98 patients with non-specific low back pain who received treatment in Xuzhou First People's Hospital from January 2020 to September 2022 were selected as the research objects, and were divided into control group (49 cases) and experimental group (49 cases) according to random number table method. The control group received interference electrotherapy, and the experimental group was combined with suspension training on the basis of the control group. Relevant indicators were evaluated before treatment (T0), at the end of 2 weeks (T1), 4 weeks (T2) and 3 months after treatment (T3). The visual analog scale (VAS), Oswestry Lumbar Dysfunction Index (ODI), Japanese Orthopaedic Association low back pain (JOA) score, and lumbar active range of motion (AROM) were compared between the two groups at different time. Results The VAS scores at T1, T2, and T3 in both groups were lower than those at T0 (P＜0.05), and the VAS scores at T1, T2, and T3 in the experimental group were lower than those in the control group (P＜0.05). There was no significant difference in the ODI scores at T0 between the two groups (P＞0.05). The ODI scores at T1, T2, and T3 in the two groups were lower than those at T0, and the decrease in the experimental group was more significant, with a statistically significant difference (P＜0.05). There was no significant difference in JOA scores and T0 time between the two groups (P＞0.05). The JOA scores at T1, T2, and T3 times in the two groups increased compared to T0 time, and the degree of increase in the experimental group was more significant, with a statistically significant difference (P＜0.05). There was no significant difference between the two groups in terms of forward flexion, backward extension, left flexion, and right flexion at T0, T1,T2,T3 (P＞0.05).Conclusion Suspension training combined with interference electrotherapy can alleviate the pain level of patients with nonspecific low back pain and improve lumbar function, which has practical significance for promotion