Objective To explore the correlation and diagnostic value of T lymphocyte, inflammatory cytokines in peripheral blood to ovarian endometriosis (OMs). Methods From October 2020 to June 2022, 72 patients with pathologically confirmed OMs undergoing open or laparoscopic surgery were admitted to the case group, 37 patients were divided into stage I-II and there were 35 patients in stage III-IV according to the modified endometriosis stage (r-AFS). 72 patients with Simple cyst or benign teratomas after the same period of surgery were selected as control group. Flow cytometry was used to detect CD4+, CD8+, CD4+/CD8+, IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ and TNF-α in peripheral blood and gather related clinical data by univariate and logistic multivariate regression analysis to discuss the influencing factors of EMs. Patients in stage of I-II and II-IV in OMs were further compared with CD4+, CD8+ and TNF-α which were independent influencing in the peripheral blood indexes express differences. The ROC curve evaluates the diagnostic efficacy of OMs by three indicators, both individually and in combination. Results CD8+, IL-2, IL6, IL-10, TNF-α, menarche age＜13 years, menstrual period days＞6 days, dysmenorrhea, abortion surgery or cervical surgery history and caesarean section history were OMs risk factors, CD4+ and CD4+/CD8+ were OMs protection factors. CD8+, TNF-α, menstrual period＞6 days, dysmenorrhea, abortion surgery or hysteresis surgery and uterine surgery history were independent risk factors for OMs, and CD4+ was an independent protective factor for OMs. There were Significant differences between stage I-II and III-IV of OMs in CD4+, CD8+ and TNF-α (P＜0.05). The combined detection of CD4+, CD8+ and TNF-α could improve the diagnostic efficiency of OMs. Conclusion T lymphocyte subsets and inflammatory cytokines Peripheral blood play a role in the occurrence of OMs, CD4+, CD8+ and TNF-α are involved in disease progression and the combined detection of the three may have high application value in the diagnosis of OMs