Objective To investigate the expression levels o cell division cyclin (CDC6) and microchromosome maintenance protein (MCM6) protein and mRNA in cervical pain tissues and their relationship with the clinicopathologic features of cervical cancer.Methods From October 2018 to November 2020, the patients admitted to the Department of Gynecology of the Affiliated Hospital of North China University of Science and lechnology were collected as subjects. The expressions of CDC protein and MCM6 protein in 44 cases of cervical cancer and 50 cases of normal cervical tissues were detected by immunohistochemical method. Real-time quantitative fluorescence PCR (qRT-PCR) was used to detect the expressions of CDC mRNA and MCM6 mRNA in the cervical tissues of the above two groups. Results The positive expression rate of CDC6 protein and MCM6 protein in cervical cancer group was significantly higher than that in normal cervical cancer group, the difference was statistically significant (P＜0.01). The positive expression rates of CDC6 protein and MCM6 protein in cervical cancer tissues were correlated with lymph node metastasis, clinical stage and HPV16/18 infection of cervical cancer patients, and the differences were statistically significant (P＜0.05), but not related to menopause, mass size, parastatal infiltration, degree of differentiation and tissue type（P＞0.05）. The expression level of MCM6 mRNA in cervical cancer group was higher than that in normal cervical cancer group, and the difference between the two groups was statistically significant (P＜0.05). When the exoression of CDC6 mRNA or MCM6 mRNA was increased, the risk of cervical cancer was increased（OR: 2.591, 95%CI: 1.126～5.962; OR: 4.239, 95%CI:1.780～10.095）. In cervical cancer tissues with positive expression of MCM6 protein, the positive expression rate of CDC protein was higher, and the difference was statistically significant (P<0.05). Conclusion The expressions of CDC6 and MCM6 protein and mRNA are related to the occurrence and development of cervical cancer, which can be used as biological indicators of cervical cancer