基于网络药理学探讨桂枝茯苓丸治疗急性脑梗死的作用机制及体内验证
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国家中医药管理局国家中医临床研究基地业务建设科研专项课题(JDZX2015043);北京中医药大学2020年度基本科研业务(重点攻关项目)(2020-JYB-ZDGG-129)


Mechanism of Guizhi Fuling Wan in the treatment of acute cerebral infarction based on network pharmacology and vivo experiment
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    摘要:

    目的 基于网络药理学的研究方法探讨桂枝茯苓丸治疗急性脑梗死的作用机制。方法 在中药系统药理数据库及分析平台(TCMSP)数据库中收集桂枝茯苓丸的主要活性成分,设置口服利用度(OB)≥30%,类药性(DL)≥0.18为筛选条件;使用GeneCards、OMIM、DrugBank数据库收集急性脑梗死的作用靶点,获得桂枝茯苓丸与疾病的交集靶点,绘制交集韦恩图;利用BisoGenet构建PPI网络,通过Cytoscape提取核心靶点;获取关键靶点蛋白后利用Metascape进行GO功能及KEGG通路富集分析。结果 利用TCMSP检索共获得桂枝茯苓丸有效成分65个,主要为槲皮素、β-谷甾醇、齿孔酸、猪苓酸C、白花素等;筛选出桂枝茯苓丸治疗急性脑梗死的关键靶点135个,包括TP53、MCM2、UBC等;涉及细胞周期信号通路、泛素介导的蛋白降解信号通路、PI3K-Akt信号通路等119条相关信号通路;分子对接结果显示多个化合物与蛋白的结合能≤8 kJ/moL;实验组的tp53、UBC基因表达量较模型组的低,PI3K、P-Akt蛋白表达量较模型组的高(P<0.05)。结论 桂枝茯苓丸治疗急性脑梗死具有多通路、多靶点的特点,为今后研究桂枝茯苓丸治疗急性脑梗死的机制提供了科学的理论依据及研究思路

    Abstract:

    Objective To explore the mechanism of Guizhi Fuling Wan in the treatment of acute cerebral infarction based on the research method of network pharmacology. Methods The main active components of Guizhi Fuling Wans were collected in tcmsp database. The screening conditions were oral bioavailability(OB) ≥ 30% and drug-likeness (DL) ≥ 0.18. Using genecards, OMIM and drugbank databases to collect the action targets of acute cerebral infarction, obtain the intersection targets of Guizhi Fuling Wan and disease, and draw the intersection Wayne diagram. The PPI network was constructed by bisogenet, and the core targets were extracted by Cytoscape. After obtaining key target proteins, GO function enrichment analysis and KEGG analysis were analyzed by metascape. The results of network pharmacology were verified by qRT-PCR and WB. Results 65 active components of Guizhi Fuling Wan were obtained by tcmsp search, mainly Quercetin,β- Sitosterol, Eburicoic acid, Polyporenic acid C, Albiflorin, etc. 135 key targets of Guizhi Fuling Wan in the treatment of acute cerebral infarction were selected, including TP53, MCM2, UBC, etc. It involves 119 related signal pathways such as cell cycle, Ubiquitin mediated proteolysis and PI3K-Akt signaling pathway; Molecular docking results showed that the binding energies of several compounds with proteins were less than 8 kJ/mol. The expression of tp53 and UBC genes in the experimental group was lower than that in the model group. The expression of PI3K and P-Akt protein in the experimental group was higher than that in the model group. Conclusion Guizhi Fuling Wan has the characteristics of multi-channel and multi-target in the treatment of acute cerebral infarction, which provides a scientific theoretical basis and research ideas for the future study of the mechanism of Guizhi Fuling Wan in the treatment of acute cerebral infarction

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  • 在线发布日期: 2024-06-18
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