Abstract:Objective To explore the mechanism of Guizhi Fuling Wan in the treatment of acute cerebral infarction based on the research method of network pharmacology. Methods The main active components of Guizhi Fuling Wans were collected in tcmsp database. The screening conditions were oral bioavailability(OB) ≥ 30% and drug-likeness (DL) ≥ 0.18. Using genecards, OMIM and drugbank databases to collect the action targets of acute cerebral infarction, obtain the intersection targets of Guizhi Fuling Wan and disease, and draw the intersection Wayne diagram. The PPI network was constructed by bisogenet, and the core targets were extracted by Cytoscape. After obtaining key target proteins, GO function enrichment analysis and KEGG analysis were analyzed by metascape. The results of network pharmacology were verified by qRT-PCR and WB. Results 65 active components of Guizhi Fuling Wan were obtained by tcmsp search, mainly Quercetin,β- Sitosterol, Eburicoic acid, Polyporenic acid C, Albiflorin, etc. 135 key targets of Guizhi Fuling Wan in the treatment of acute cerebral infarction were selected, including TP53, MCM2, UBC, etc. It involves 119 related signal pathways such as cell cycle, Ubiquitin mediated proteolysis and PI3K-Akt signaling pathway; Molecular docking results showed that the binding energies of several compounds with proteins were less than 8 kJ/mol. The expression of tp53 and UBC genes in the experimental group was lower than that in the model group. The expression of PI3K and P-Akt protein in the experimental group was higher than that in the model group. Conclusion Guizhi Fuling Wan has the characteristics of multi-channel and multi-target in the treatment of acute cerebral infarction, which provides a scientific theoretical basis and research ideas for the future study of the mechanism of Guizhi Fuling Wan in the treatment of acute cerebral infarction