肿瘤相关巨噬细胞外泌体调控肝癌细胞生长、转移及衰老的功能
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首都临床特色应用研究项目(Z181100001718143)


Study on the function of tumor-associated macrophage exosomes in regulating the growth, metastasis and senescence of hepatocellular carcinoma cells
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    目的 探讨肿瘤相关巨噬细胞外泌体对肝癌细胞生长、转移及衰老的影响及机制。 方法 提取并鉴定M0和M2型巨噬细胞外泌体(M0 exo和M2 exo),肝癌细胞HepG2和SMMC-7721细胞分为PBS组、M0 exo组和M2 exo组,CCK8法检测各组肝癌细胞的增殖能力,Transwell法检测各组细胞迁移和侵袭能力,SA-β-Gal染色检测细胞衰老,流式细胞术检测细胞周期,Western blot检测各组细胞P16、P21、HMGA1的表达水平。〖HTH〗结果 M0 exo和M2 exo均符合外泌体的结构和生物学特征,相较于PBS组,M2 exo组HepG2和SMMC-7721细胞增殖能力显著增加,迁移和侵袭能力显著增加,细胞周期G1期降低,SA-β-Cal染色阳性率显著降低,衰老相关蛋白P16、P21、HMGA1表达显著下调(均P<0.05),而M0 exo组上述指标与PBS组比较,差异均无统计学意义(P>0.05)。结论 M2型巨噬细胞外泌体可诱导肝癌细胞HepG2和SMMC-7721增殖、迁移和侵袭能力的增加,其机制可能为抑制细胞衰老

    Abstract:

    Objective To investigate the effect and mechanism of tumor related macrophage exoosomes on the growth and metastasis ability of liver cancer cells. Methods The exosomes of M0 and M2 macrophages (M0 exo and M2 exo) were extracted and identified. HepG2 and SMMC-7721 cells were divided into PBS group, M0 exo group and M2 exo group. CCK8 method was used to detect the proliferation ability of hepatoma cells in each group, Transwell method was used to detect the migration and invasion ability of cell migration, flow cytometry was used to detect the cell cycle, SA-β-Gal staining was used to detect the Cellular senescence, and Western blot was used to detect the expression level of P16, P21, HMGA1. Results Both M0 exo and M2 exo conformed to the structure and biological characteristics of exosomes. Compared with PBS group, the proliferation, migration and invasion ability of HepG2 and SMMC-7721 cells in M2 exo group was significantly increased, the G1 of cell cycle was increased, cellular senescence significantly reduced, the expression of aging related proteins P16, P21, and HMGA1 were significantly downregulated (P<0.05) Conclusion M2 type macrophage exosomes can promote the proliferation, migration, invasion abilities and cell senescence of HepG2 and SMMC-7721

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  • 在线发布日期: 2024-06-18
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