Objective To study the diagnostic value of high-sensitivity C- reactive protein (hs-CRP), serum procalcitonin (PCT) and white blood cell count (WBC) in newborns with infectious diseases, so as to provide evidence for reducing the antibiotic utilization empirically and neonatal mortality. Methods 62 newborns with infectious diseases admitted to our hospital from January 2021 to December 2021 were the case group, and the 50 newborns with non infectious diseases admitted in the same department in the same period were taken as the control group. Venous blood was collected on the 1st day and the 7th day of admission to observe the difference of hs-CRP, PCT and WBC count in the two groups, calculate the sensitivity and specificity of hs-CRP, PCT and WBC, and draw the ROC curve. Results The hs-CRP, PCT and WBC counts of the case group were higher than those of the control group at admission,and the difference was statistically significant(P＜0.001). On the 7th day after admission, hs-CRP and PCT in the case group decreased significantly, which was not statistically significant compared with the control group(P＞0.05) Although WBC count decreased significantly, but it was still higher than that in the control group (P＜0.05). The area under ROC curve of hs-CRP, PCT, WBC count and hs-CRP + PCT in the diagnosis of neonatal infectious diseases were 0.954, 0.962, 0.732 and 0.985, respectively.The Yodon index of hs-CRP+PCT was the highest, and the area under the curve was the largest, followed by PCT, hs-CRP, and WBC. The differences were statistically significant（P＜0.05）.Conclusion Hs-CRP, PCT and WBC counts have certain diagnostic value in the early stage of neonatal infectious diseases. Hs-CRP+PCT is helpful to decide whether to use antibiotics