Objective To observe the effect of interfering INHBA-AS1 on inhibiting the proliferation and promoting the apoptosis of cervical cancer cell Hela through glycolysis, and explore its possible mechanism. Methods Hela cells were divided into blank control, negative control, INHBA-AS1 shRNA, Prosapogenin A (10 μM, 48 h), negative control+Prosapogenin A (10 μM, transfection after 48 h of treatment), and INHBA-AS1 shRNA + Prosapogenin A (10 μM, transfection after treatment). Cell proliferation was detected by CCK8. Cell apoptosis was detected by TUNEL. Glucose, lactic acid and ATP test boxes were used to detect the content. The mRNA expressions of INHBA-AS1, HK2, PDK1, GLUT1 and LDHA were detected by qRT-PCR, and the protein expressions of HK2, PDK1, GLUT1 and LDHA were detected by Western blot. Results Compared with the control group, the proliferation of the cells in INHBA-AS1 shRNA or Prosapogenin A group was inhibited (P＜0.05), and the apoptosis was promoted. The content of glucose was increased significantly (P＜0.05), while the content of lactic acid and ATP was decreased markedly (P＜0.05). The mRNA and protein expression levels of HK2, PDK1, GLUT1 and LDHA was decreased significantly (P＜0.05). Meanwhile, treatment of cells with INHBA-AS1 shRNA and Prosapogenin A aggravated the above phenomenon (P＜0.05).Conclusion Interfering with INHBA-AS1 can inhibit the proliferation and promote the apoptosis of cervical cancer cell Hela by inhibiting glycolysis, and its mechanism may be related to PDK1