Objective To analyze the curative effect of transverse tibial bone transport on diabetic foot and its influence on microcirculation. Methods A total of 85 patients with diabetic foot of Wagne grade III or above who were admitted to Osteopathy Center, Dazhou Hospital of Integrated Traditional Chinese and Western Medicine from December 2016 to January 2021 were selected. All underwent transverse tibial bone transport. The perioperative indexes and postoperative wound healing of affected foot were evaluated. The curative effect of transverse tibial bone transfer on diabetic foot was verified by self-control method. The temperature of foot skin, pain, lower limb peripheral neuropathy, foot blood flow indexes, levels of angiogenesis-related factors and wound microcirculation were compared before and after operation. Results All patients successfully completed the operation. The operation time and blood loss were (56.78±8.25) min and (35.49±6.13) mL, respectively. After operation, there were 2 cases with incision flap hematoma which healed after hematoma removal and pressure bandaging. No complications such as skin necrosis, infection, fracture or external fixator loosening were observed. Postoperative followed up found that 1 week after bone transport, there were fresh granulation tissues on ulcer wound of the affected foot. 3-4 weeks later, the area of ulcer wound was significantly reduced, and started healing 6-10 weeks later, with average healing time of (12.35±2.37) weeks. There was 1 case undergoing necrotic toe amputation at 2 weeks after operation, with a limb salvage rate of 97%. There were 3 cases with foot ulcer recurrence within 1 year after operation, with a recurrence rate of 13.04%. The VAS scores, TCSS scores and mean flow velocity were reduced at 3 months after operation (P＜0.05). The temperature of foot skin, lumen diameter ofdorsalis pedis artery, serum VEGF and EGF levels, partial pressure of percutaneous oxygen and blood perfusion volume were increased (P＜0.05). The change rate of blood perfusion volume was (24.04±5.36)%. Conclusion Transverse tibial bone transport is effective in the treatment of diabetic foot, which can accelerate wound healing and reduce amputation rate by promoting wound microcirculation