The kidney is one of the important organs in the occurrence of ischemia-reperfusion injury (IRI). Renal IRI is the main cause of acute kidney injury (AKI). At present, many in vivo and in vitro experimental models have been used to study the pathogenic mechanism and renal protection of ischemic AKI. Among them, due to the characteristics of small size, convenient feeding, good controllability, and fast reproduction of mice, the method of establishing renal IRI models by clipping mouse renal pedicles is widely used. However, it is not easy to establish a good and stable mouse kidney IRI model, because mice are extremely sensitive to external stimuli, which often leads to certain differences in experimental results. Therefore, this paper reviews the experience and progress of the establishment of the bilateral renal IRI mouse model, and based on this, it provides a certain reference value for the establishment of a standard renal IRI model, and also for other researchers to establish a good and reliable renal IRI mouse model provide help