Objective To screen lentiviral vector of runt-related transcription factor2(Runx2) gene to transfect GNM cells with cervical lymph node metastasis from human maxillary gingival carcinoma and obtain the silencing of Runx2 gene expression in GNM cell, which laid the experimental foundation for the later study on the transcriptional regulation of Runx2 in GNM cells. Methods Based on the preliminary results, in the culture medium containing Hitransg P, virus were transfected with GNM cells according to multiplicity of infection (MOI) 10 and viruses were divided into four groups according to their serial number :NC :CON313; KD1: LV-Runx2-RNAi(80978-1); KD2: LV-Runx2-RNAi(80979-1); KD3: LV-RUNX2-RNAi (80980-1), fluorescence microscopy was used to observe the expression of fluorescence marker genes in each group 120 h after transfection. Real-time PCR was used to analyze the transcription product level of Runx2 and compare the silencing efficiency of Runx2 gene between groups. Western Blot further detected the protein silencing effect of Runx2 gene. Results After 120 hours of lentivirus transfection, the cells in each group were in good condition. The fluorescence expression was not observed in NC group. The fluorescence expression was higher in KD1 group. The fluorescence expression in KD2 group was the highest. The fluorescence expression was lower in the KD3 group. Real-time PCR analysis showed that the best silencing effect of Runx2 gene was in the KD2 group. The silencing efficiency was up to 64.4%（P<0.05). Western Blot results showed that Runx2 protein expression of GNM cells in the KD2 group was the lowest among all experimental groups（P<0.05). Conclusion The lentiviral vectors specifically transfected GNM cells are successfully screened out, and relevant appropriate parameters of transfection experiment are preliminarily determined.