Objective To study the effects of LY294002 on oxygen free radical, TRPV1 neuronal sensitivity and TSLP expression in allergic rhinitis-asthma syndrome rats. Methods Forty rats were randomly divided into healthy group (healthy rats were routinely fed), model group (model of allergic rhinitis asthma syndrome was established by ovin sensitization and nasal drip attack), treatment group (model+intravenous injection of LY294002 0.5 mg/kg), and control group (model+budesonide aerosol). The expression of TSLP was detected by ELISA and immunohistochemistry, SOD was detected by xanthine oxidase, MDA was detected by TBA, histopathological changes of lung tissues were observed by HE staining, the expression of TRPV1 was detected by immunofluorescence, and the expressions of NF-кB and IкB were detected by western blot. Results Compared with the healthy group, there were a lot of inflammatory cells infiltration, bronchial mucosa edema and thickening, lumen narrowing and mucus secretion increased in model group. Compared with model group, the pathological changes of lung tissue in treatment group and control group were significantly improved. Compared with the healthy group, TRPV1, MDA, TSLP and NF-кB were increased, while SOD and IкB were decreased in the model group (P<0.05). Compared with the model group, TRPV1, MDA, TSLP and NF-кB were decreased in the treatment group and the control group, while SOD and IкB were increased (P<0.05). There was no difference in the indexes between the treatment group and the model group (P>0.05). Conclusion By inhibiting TRPV1, TSLP and MDA and promoting the expression of SOD, the neurogenic inflammation of airway in CARAS rats is reduced, the generation of airway hyperreactivity is prevented and the level of oxidative stress injury and pathology is improved, thus playing a therapeutic role.