To analyze the expressions of long non-coding RNA (lncRNA) H19 and silent information regulator 6 (SIRT6) in human gastric cancer tissues and their correlation with clinicopathology.Methods 96 patients with gastric cancer were selected as the research subjects, and the specimens of gastric cancer tissues and adjacent normal tissues (＞5 cm from the gastric cancer tissues) were collected.The expressions of lncRNA H19 and SIRT6 in human gastric cancer tissues and adjacent normal tissues were compared, and the relationship between the expressions of lncRNA H19 and SIRT6 and clinicopathological characteristics of patients with gastric cancer was analyzed.Results The expression level of lncRNA H19 mRNA in human gastric cancer tissues was significantly higher than that in adjacent normal tissues (P＜0.05).The expression level of SIRT6 mRNA and positive expression rate of SIRT6 protein in human gastric cancer tissues were significantly lower than those in adjacent normal tissues (P＜0.05).The expression level of lncRNA H19 mRNA was positively correlated with the degree of differentiation, depth of invasion, tumor TNM stage and lymph node metastasis in patients with gastric cancer (P＜0.05).The expressions of SIRT6 mRNA and SIRT6 protein in patients with gastric cancer were significantly negatively correlated with the differentiation degree, invasion depth, tumor TNM stage and lymph node metastasis (P＜0.05).Conclusion The expression of lncRNA H19 is up-regulated in human gastric cancer tissues while the expression of SIRT6 is down-regulated.There is a certain correlation between the expressions of lncRNA H19 and SIRT6 and the clinicopathological characteristics of patients with gastric cancer