The network pharmacology and molecular docking technology were applied to explore the active components, potential targets and the mechanism of Yiqi Huoxue Prescription on treating Diabetic Nephropathy.It aims to put forward new treatment ideas for Diabetic Nephropathy (DN).Methods Multiple public databases were used to select the active compounds and their potential targets of Yiqi Huoxue Prescription and the disease targets of DN.The intersection targets of the two were obtained to perform GO and KEGG analysis, PPI analysis and network construction.Finally, molecular docking studies were carried out on key components and targets by using Autodock Vina software.Results A total of 78 active components and 223 potential targets of Yiqi Huoxue Prescription, 1002 disease targets of DN, 81 intersection targets and 15 key targets were obtained by network pharmacology analysis.The results of KEGG pathway included insulin resistance, PI3K-Akt signaling pathway and so on.Molecular docking results showed that kaempferol, Luteolin and other components had affinity for most of the targets.Conclusion Yiqi Huoxue Prescription may treat DN by regulating inflammation and autophagy, promoting the degradation of ECM protein, regulating the sensitivity of peripheral tissues to insulin and the proliferation and apoptosis of pancreatic β cells.But further experimental research and verification are still needed