To explore the clinical value and influencing factors of arterial velocity index (AVI) and arterial pressure-volume index (API), a new index of arterial stiffness. Methods 2677 volunteers were recruited from the hospital and divided into 929 patients ≥64.2 years old (Q1 group), 890 patients ≥47.8 years old (Q2 group), and 858 patients younger than 47.8 years old (Q3 group) according to the age tertiles. The AVI and API of the subjects were monitored by the cuff oscillatory wave analysis method. 124 subjects were randomly selected to obtain their left ventricular ejection fraction (EF). One-way analysis of variance was used to compare the differences in AVI and API between the three groups. Pearson correlation and multiple linear regression analysis were used to obtain independent influencing factors of AVI and API. Results The differences between AVI and API in the three groups had statistical significance (all P＜0.05), and the values of AVI and API increased with age (all P＜0.05). Elevated AVI was positively correlated with age, body mass index, systolic blood pressure and diastolic blood pressure (r=0.536, 0.055, 0.486 and 0.189, all P＜0.01), and was negatively correlated with height, body mass, ejection fraction and shortening fraction(r=-0.123, -0.049,-0.268 and -0.259, all P＜0.05). Multiple linear regression analysis indicated that age and systolic blood pressure were independent influencing factors of AVI (P＜0.05). Elevated API was positively correlated with age, body mass, body mass index, and systolic blood pressure (r=0.475, 0.108, 0.230, and 0.690, all P＜0.01), and negatively correlated with height (r=-0.107, P＜0.01) ). Multiple linear regression analysis indicated that diastolic blood pressure and systolic blood pressure were independent factors affecting API (P＜0.01). Conclusion AVI and API are new indicators reflecting arterial stiffness, which are related to age, height, body mass index and blood pressure, providing new substitute indicator for arterial stiffness screening