To investigate the psychological stress of medical workers after occupational exposure. Methods This was a prospective study. Through online questionnaire survey on medical staff with occupational exposure in a tertiary hospital in Sichuan Province from November 1, 2018 to October 31, 2020, PTSD-SS score of different gender,education background, post, exposure mode and protection situation were compared, so as to understand the psychological trauma stress of different medical staff after occupational exposure. Results During the period from November1, 2018 to October 31, 2020, a total of 447 cases of occupational exposure occurred in our hospital, including 177 males (39.60%) and 270 females (60.40%), with an average age of (28.54±7.05) years and an average length of service of (5.06±5.62) years. According to the classification of different occupations, there were 286 doctors (63.98%), 140 nurses (31.32%) and 21 administrative and logistic staff (4.70%). The highest PTSD-SS score was 120, the lowest was 24, and the average PTSD-SS score was (47.72±21.68). The average total PTSD-SS score of 447 exposed persons was (47.72±21.68), of which the highest score was 120 and the lowest score was 24. Except that there were significant differences in PTSD-SS scores among exposed persons with different exposure modes (acupuncture exposure vs. mucosal exposure), there were no significant differences in the total scores of other subgroups such as gender, education background, awareness of exposure sources and standard protection (P＞0.05). Conclusion After occupational exposure, medical personnel may have psychological stress disorders of different severity, especially those with mucosal exposure. We should pay enough attention to early prevention, early detection, early guidance and intervention to avoid serious psychosomatic damage.