To explore the effect of Chuzhen on lactation symptoms and Serum Prolactin of Cesarean Section women. Methods A total of 123 cases of pregnant women with lactation after cesarean section in The Affiliated Hospital of Chengdu University of TCM from August 2020 to February 2021 were randomly divided into the control group(61 cases) and the study group(62 cases). The control group was treated with routine treatment. The test group implemented the Chuzhen on the basis of evidence in the control group. Once a day for 5 consecutive interventions, Using RMANOVA to compare the milk yield, breast filling, milk deposition and milk viscosity of patients before intervention and after 1, 2, 3, 4 and 5 days of intervention. The Serum lactogen was compared between the two groups before and after the intervention. Results Comparison of the scores of lactation symptoms:there was no significant difference between the test group and the control group at 2 days before intervention (P＞0.05), but there was significant difference between the two groups from the third day (P＜0.05). The intra-group comparison of breast filling at different time points showed that there was no significant difference between the experimental group and the control group on the first day before intervention (P＞0.05), but there was significant difference in the second day (P＜0.05). The intra-group comparison of milk siltation degree at different time points showed that there was no significant difference between the experimental group and the control group in the first 2 days of intervention (P＞0.05), but there was significant difference in the third day group (P＜0.05). The intra-group comparison of milk viscosity at different time points showed that there was no significant difference between the experimental group and the control group 3 days before intervention (P＞0.05), but there was significant difference between the experimental group and the control group from the 4th day (P＜0.05). The intergroup effect, time effect and intergroup and time interaction effect of milk yield, breast filling degree, milk deposition degree and milk viscosity in both groups were statistically significant (P＜0.05). After intervention, serum prolactin in experimental group was higher than that in control group (P＜0.05). Conclusion Chuzhen can improve milk yield, increase breast fullness, improve the degree of milk stasis, increase milk consistency, and promote serum lactogen secretion in mothers with post cesarean deficiency.