To explore the effects of multi-point matrix scanning laser and multi-wavelength laser combined with conbercept in the treatment of diabetic retinopathy combined with DME. Methods The clinical data of 72 patients with DR combined with DME admitted to our hospital from May 2018 to May 2021 were selected and divided into the control group and the study group with 36 cases each according to the random double blind method. The control group was treated with multi wavelength laser combined with conbercept , and the research group was treated with multi point matrix scanning laser combined with conbercept . The difference in curative effect between the two groups was observed. Results The results of the analysis of the generalized estimating equation showed: Wald x2=1.272, P=0.259 between groups, suggesting that there was no difference in the effective rates of the two groups of patients after treatment for 1 month, 2 months of treatment, and 3 months of treatment (P＞0.05). In terms of time point, Wald x2=74.900, P＜0.01, suggesting that there was a significant difference in the treatment efficiency of patients at different time points (P＜0.05). Repeated measures analysis of variance showed that in terms of between groups, the thickness of RNFL in the study group was lower than that in the control group after 3 months of treatment (P＜0.05). In terms of time point, after treatment for 1 month and 3 months, the two groups of BCVA, NOS, SHBG The levels were higher than before treatment, and the levels of CMT, TMV, VEGF, IGF-1, and EPO were lower than before treatment (P＜0.05). Conclusion For patients with DR combined with DME, there is no difference in the effect of multi-point matrix scanning laser combined with conbercept and multi wavelength laser combined with conbercept . Both can improve the patient’s vision, but multi-wavelength laser treatment can induce RNGL thickening in a short period of time. Need to cause clinical attention.