Objective To observe the clinical efficacy of self-made Dizhong Gujian decoction combined with calcium carbonate D3+calcitriol in the treatment of osteoporosis (OP) of kidney deficiency and blood stasis syndrome. Methods 172 patients with OP of kidney deficiency and blood stasis syndrome treated in the hospital from May 2020 to May 2021 were divided into combined group and control group according to the simple random number table method and random remainder grouping method, with 86 cases in each group. Patients in the control group were treated with oral calcium carbonate D3+calcitriol, and patients in the combined group were combined with self-made Dizhong Gujian decoction on the basis of the control group, and both groups were treated for 3 months. The clinical efficacy, and scores of TCM syndromes (low back stabbing pain, soreness and weakness of waist and knees, weak wilting lower limbs, difficulty in walking), bone metabolism markers ［bone gla protein (BGP), bone alkaline phosphatase (BALP), type I collagen C-terminal peptide (CTX-I), tartrateresistant acid phosphatase 5b (TRACP-5b)］ and bone mineral densities (BMD) of different parts (femoral neck, femoral trochanter, lumbar spine L1-4, Ward triangle) before treatment and after 3 months of treatment were compared between the two groups of patients. Results After 3 months of treatment, the total clinical effective rate of combined group was significantly higher than that of control group (P＜0.05). The scores of TCM syndromes and levels of BALP, CTX I and TRACP-5b of the two groups were lower than those before treatment, and the scores and levels were lower in combined group than those in control group (P＜0.05). The BGP level of the two groups of patients was significantly higher than that before treatment, and the level in combined group was higher than the control group (P＜0.05). The bone mineral densities of different parts of the two groups were significantly enhanced compared to before treatment (P＜0.05), and the bone mineral densities in combined group were higher compared with those in control group (P＞0.05). Conclusion Self-made Dizhong Gujian decoction combined with calcium carbonate D3+calcitriol can effectively improve the clinical symptoms and bone metabolism level and enhance the bone mineral density of patients with OP of kidney deficiency and blood stasis syndrome, with a significant clinical efficacy.