Objective To investigate the application of multiple clinical intervention paths based on the concept of enhanced recovery after surgery (ERAS) in perioperative period of endoscopic retrograde cholangio pancreatography (ERCP) in patients with cholangiolithiasis of different ages. Methods The clinical data of 161 cholangiolithiasis patients undergoing ERCP in the First Hospital of Lanzhou University from January 2019 to December 2019 under the path were retrospectively analyzed, including 73 patients under 65 years old (lower age group) and 88 patients over 65 years old(higher age group). Results ERCP was successfully performed in all patients and there were no statistically significant differences in white blood cells, c-reactive protein, heart rate and mean arterial pressure between preoperation and postoperation (P＞0.05). The mean operative time was (4080±2983)min. The average time to get out of bed was (9.34±6.04)h, the average time to eat for the first time was (17.57±13.33)h, the average time to sleep at night was (7.53±1.42)h, and the average postoperative hospital stay was (4.13±3.31) days. The most common postoperative complications were abdominal distension (65.2%), nausea (20.4%) and cholangitis (9.9%), and those were no statistical significance in different ages. Multivariate Logistic regression shows preoperative fasting time, operative time, postoperative first feeding time, postoperative length of stay were correlated with postoperative complications(P＜0.05). Conclusion The application of multiple clinical intervention paths based on ERAS in the perioperative period of ERCP for cholangiolithiasis patients is safe and effective in different ages, which plays an important role in promoting the recovery after surgery, reducing perioperative stress and reducing the incidence of complications, and is worthy of being popularized in clinical practice.