Objective To provide the reference values of adrenal measurement parameters in healthy adults, explore the relationship between adrenal volume (AGV) and anthropometric parameters, and establish an AGV prediction model. To analyze the relationship between AGV and adrenal hormones, blood pressure, fasting blood glucose (FBG), blood lipid and uric acid (UA) in healthy adults.Methods 105 healthy volunteers (HVs) randomly recruited in Chengdu from 2018 to 2019 were included. Age, sex, height, weight, waistline, body mass index (BMI), body surface area (BSA), blood pressure and other information of them were collected. FBG, blood lipids, UA and adrenal hormones were measured in the laboratory department of West China Hospital of Sichuan University, and adrenal CT scan was performed. Based on medical digital imaging and communication (DICOM) images, the contour of adrenal gland was depicted manually, and the AGV was calculated by semi-automatic volumetric method. SPSS 23 statistical software was used for statistical analysis.Results The 95% reference range of left adrenal mean limb width (ALW) and right ALW were 0.27-0.57cm and 0.22-0.49cm, respectively. The 95% reference ranges of left AGV (L-AGV), right AGV (R-AGV) and total AGV (T-AGV) were 1.86-5.08 cm3, 1.21-4.36 cm3 and 3.30-9.21cm3, respectively. 95% reference values of left and right ALW ratio (L/Rw) and left and right adrenal volume ratio (L/ Rv) were 0.76-1.66 and 0.73-1.91, respectively. Male T-AGV was greater than female (P＜0.0001). Including demographic parameters and adrenal measurement parameters, the prediction model of AGV was established by multiple linear stepwise regression analysis, L-AGV (cm3)=0.199*length (cm)+0.495*height (cm)+3.359*ALW (cm)+0.037*body weight (kg)-2.128, adjusted R2 was 0.663; R-AGV (cm3)=0.414*length (cm)+0.552*height (cm)+3.595*ALW (cm)+0.024*body weight (kg)-2.857, adjusted R2 was 0.579. T-AGV was positively correlated with 8 a.m. cortisol (PTC), adrenocorticotropin (ACTH), 24-hour urinary free cortisol (24hUFC), norepinephrine, systolic blood pressure (SBP), diastolic blood pressure (DBP) and UA. After weight correction, the correlation between T-AGV and those biochemical indexes was disappeared. There was no significant correlation with renin activity (PRA), aldosterone (ALD), FBG, triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) and serum potassium.Conclusion This study established the reference range of adrenal measurement parameters, providing baseline data for the follow-up exploration of the application of adrenal measurement in the diagnosis and treatment of adrenal diseases. T-AGV was closely related to body weight, and the influencing factors of body weight should be considered in the analysis of adrenal measurement values.