Objective By monitoring the cleavage rate and blastocyst rate in vitro mouse embryo culture experiments, we evaluate whether the new reproductive embryo laboratory of our hospital meets the requirements of in vitro human embryo culture. Methods In vitro and in vivo fertilization experiments were performed on SPF Kunming mice. Female mice were intraperitoneally injected with 10 U PMSG per mouse.48 h later, intraperitoneal injection of hCG 10 U/ mouse was performed for ovulation induction. After ovulation induction, in vitro fertilization group underwent surgery to obtain sperm and egg for in vitro fertilization culture. In the in vivo fertilization group, after ovulation induction in female mice, female mice and male mice were caged together overnight, and the female mice with pubic supposit were killed in the morning of the next day, and the fertilized prokaryotic embryos were obtained by operation for culture, and the development of embryos in the two groups during the culture was observed. Results A total of 946 eggs were obtained after 20 cycles of in vitro fertilization. The fertilization rate and blastocyst rate were (79.02±4.05)% and (80.12±5.27)%，respectively. In vivo fertilization experiment was carried out for 14 cycles, and 618 eggs were obtained. The fertilization rate and blastocyst rate of the two groups were (80.65±4.22) % and (81.35±3.06) %. There was no significant difference between the two groups (P>0.05). Conclusion Through monitoring the cleavage rate and blastocyst rate of mouse embryo culture in vitro to evaluate the laboratory environment of reproductive embryos in our hospital, the results show that it meets the quality control standards of all indicators.