Objective To explore the expression of LncRNA FOXD3-AS1 in ovarian cancer SKOV3 cells and the mechanism of its influence on the malignant biological behavior of SKOV3 cells. Methods Fifty-five patients with ovarian cancer diagnosed clinically in the department of pathology of our hospital from October 2020 to June 2021 were collected. The expression of FOXD3-AS1, miR-325 and CDCA5 in ovarian cancer tissues, paracancer tissues, SKOV3 cells and IOSE80 cells were analyzed by RT-qPCR. The expression of FOXD3-AS1 and CDCA5 was knockdown by siRNA, the proliferation, migration and EMT of SKOV3 cells were detected by cell cloning formation assay, cell migration assay and Western blot. The relationships between LncRNA FOXD3-AS1 and miR-325, miR-325 and CDCA5 were analyzed by miRanda, TargetScan and dual luciferin reporter assay. The proliferation, migration and EMT of SKOV3 cells were analyzed after transfection with miR-325 inhibitor or co-transfection with pcDNA-FoxD3-AS1 and miR-325 mimics.Results Compared with IOSE80 cells and paracancer tissues, the expression of LncRNA FOXD3-AS1 and CDCA5 in SKOV3 cells and ovarian cancer tissues was up-regulated (P＜0.01), and the expression of miR-325 was down-regulated (P＜0.01), siRNA knockdown of FOXD3-AS1 and CDCA5 inhibited SKOV3 cell proliferation, migration and EMT. The downstream target of LncRNA FOXD3-AS1 is miR-325, and the target of miR-325 is CDCA5.After co-transfection with miR-325 mimics, pcDNA-FOXD3-AS1 partially reversed the inhibitory effect of miR-325 mimics transfected cells on proliferation, migration and EMT of SKOV3 cells. Conclusion LncRNA FOXD3-AS1 expression was up-regulated in ovarian cancer SKOV3 cells and affected the malignant biological behavior of SKOV3 cells through miR-325 /CDCA5 axis.