Objective To explore the effect of inhibiting chemokine like factor-1 (CKLF-1) on allergic rhinitis (AR) in rats with interleukin-9 (IL-9) Influence with eosinophils. Methods 40 SD rats were randomly divided into control group (Control), model group (AR), CKLF1 antibody group (AR+CKLF1-C19), fluticasone propionate group (AR+FP), 10 rats in each group. Except for the control group, the rats in the other groups were all induced AR with ovalbumin (OVA) and aluminum hydroxide ［Al(OH)3］ sensitization method, during the modeling period, the AR+CKLF1-C19 group was instilled 5 μL of CKLF1-C19 peptide in the bilateral nasal cavity for 10 consecutive days before AR challenge; in the AR+FP group, 5 μL fluticasone propionate spray were dripped into the bilateral nasal cavity for 10 consecutive days before AR challenge. Both the control group and the model group were instilled 5 μL of saline in both nasal cavities at the same time. After the last nasal cavity challenge, rats in each group were scored with apparent behavior indicators. ELISA method was used to detect the level of serum IL-9, immunoglobulin E (IgE), interferon-γ (IFN-γ). HE staining was used to detect the expression of eosinophils in nasal mucosa tissue. Wright staining was used to detect the number of eosinophils in the nasal cavity. Immunohistochemical staining was used to detect the expression of IL-9 in nasal mucosa tissue. Real-time fluorescent quantitative PCR and Western blot was used to detect eosinophil major basic protein (MBP), eosinophil cationic protein (ECP), and eosinophil peroxidase in nasal mucosa tissue (Eosinophil peroxidase, EPO) expression at the mRNA and protein levels. Results Compared with the control group, rats in the model group had sneezing, runny nose and frequent snatching. The serum IL-9 and IgE levels increased, the IFN-γ level decreased, and the nasal mucosa had edema and thickened mucosal layer. As well as the symptoms of mucosal epithelial shedding, the number of eosinophils increased, the positive expression of IL-9 in nasal mucosa tissue increased, and the expression of MBP, ECP, and EPO at the mRNA and protein levels were all up-regulated (P＜0.05). Compared with the model group, rats in the AR+CKLF1-C19 group and AR+FP group slowed down their sneezing, runny nose, and snatching behaviors, serum IL-9 and IgE levels decreased, IFN-γ levels increased, and nasal mucosa edema. And the infiltration of inflammatory cells was reduced, the number of eosinophils decreased, and the positive expression of IL-9 in nasal mucosa tissue decreased. At the same time, the expressions of MBP, ECP, and EPO at the mRNA and protein levels were all down-regulated (P＜0.05). Conclusion Inhibiting the expression of CKLF-1 in the rat model of allergic rhinitis can significantly reduce the infiltration of eosinophils and inhibit the expression of IL-9, thus playing a certain therapeutic effect on AR.