Objective This paper investigated the effect of galangin-induced macrophage polarization on tumor volume and zinc finger protein Zbed3 in rats with liver cancer. Methods Seventy-four healthy male SD rats aged 6 to 8 weeks with body weight of 250 to 300 g were fed conventionally with free diet and water. The parameters were randomly divided into control group(normal feeding of healthy rats, n=14), model group(model rats were reared routinely, n=13), low dose of galangin(model+25 mg/kg/d galangin, n=13), medium dose group of golangin(model+50 mg/kg/d golangin, n=13), high-dose group of golangin(model+100 mg/kg/d golangin, n=13). HE staining was used to detect the pathological morphology of liver tissue. Apoptosis of hepatoma cells was detected by TUNEL. Tumor volume was measured with vernier calipers. The expression of CD11c and CD206 was detected by immunohistochemistry. The levels of Zbed3, GSK3β, Axin, β-catenin, C-myc and cyclin-D1 were detected by Western Blot. Results Rats in control group had clear hepatic lobule structure and normal liver cells. The tumor tissue of the model group showed dilatant growth, cancer cells were of different sizes, and the cords of liver cells were extruded and disarranged, with fibrinlike structure. In low, medium and high dose galangin groups, tumor cell space was widened, tumor cells showed swelling and foamy changes to varying degrees, tumor cell nests were infiltrated by a large number of inflammatory cells, and tumor cell necrosis foci of varying degrees were observed in all groups. Compared with control group, CD11c, GSK3β and Axin in model group were decreased, and CD206, Zbed3, β-catenin, C - YMC and cyclin-D1 were increased(P＜0.05). Compared with model group, apoptosis rate, CD11c, GSK3β and Axin were increased, and tumor volume, CD206, Zbed3, β-catenin, C-YMC and cyclin-D1 were decreased in GF group(P＜0.05). Compared with low dose group, apoptosis rate, CD11c, GSK3β and Axin were increased, and tumor volume, CD206, Zbed3, β-catenin, C-YMC and Pressume cyclin-D1 were decreased in medium dose group(P＜ 0.05). Compared with medium dose group, apoptosis rate, CD11c, GSK3β and Axin were increased in high dose group, and tumor volume, CD206, Zbed3, β-catenin, C-YMC and cyclin-D1 were decreased(P＜0.05). Conclusion By regulating the Wnt/β-catenin pathway, galangin promoted the transformation of macrophages into M1-type and inhibited the transformation of macrophages into M2-type, and reduced the level of Zbed3, thus promoting the apoptosis of cancer cells and reducing the tumor volume.