Objective To explore the effect and possible mechanism of PSSC on H22 Tumor bearing mice with hepatoma ascites. Methods Eighty BALB/ C mice were selected to establish H22 tumor-bearing mice model, and randomly divided into 5 groups: control group, cyclophosphamide group(CTX, 20 mg/kg), PSSC group(20, 40 and 120 mg/kg), with 16 mice in each group. Abdominal circumference, weight, survival rate and survival time of mice in each group were observed. T and B lymphocyte proliferation were detected by spleen lymphocyte transformation test. T cell immune function was observed by E- rosette experiment. The concentrations of IL-2, γ- interferon, TNF-α and VEGF in serum and ascites were detected by ELISA. Apoptosis of H22 cells was detected by TUNEL assay. The activities of Caspase-3 and Caspase-8 were determined by Caspase- colorimetry. The expressions of Janus kinase 1(Jak1), signaling and transcription activator -3(Stat3), Bcl2- related X protein(Bax) and B lymphocytoma -2(Bcl-2) were detected by Western blot. Results Compared with the control group, the abdominal circumference and body weight of PSSC group decreased significantly, while the survival rate and survival time increased in a dose-dependent manner; Compared with CTX group, PSSC 40 and 120mg/kg groups decreased abdominal circumference and body weight, but increased survival rate and survival time(P＜0.05). Compared with the control group, SI and EtRFC in PSSC group increased with the increase of PSSC treatment dose; Compared with CTX group, the percentage of SI and EtRFC in PSSC 120mg/kg group increased(P＜0.05). Compared with the control group, the levels of IL-2, IFN-γ and TNF-α in serum and ascites of H22 Tumor Mice in PSSC group increased in a dose-dependent manner, while the expression of VEGF decreased. Compared with CTX group, the expressions of IL-2, IFN-γ and TNF-α in serum and ascites of mice in PSSC 120 mg/kg group were increased, while the expression of VEGF was decreased. TUNEL showed that PSSC increased the apoptosis of H22 cells in a dose-dependent manner compared with the control group. Compared with the control group, the expression of Caspase-3 and caspase-8 increased with the increase of PSSC concentration. The results of westerblot showed that the expression of Jak1, STAT3 and bcl-2 protein in PSSC group was lower than that in control group, while the ratio of Bax/Bcl-2 was higher. Conclusion PSSC has anti-tumor effect on H22 Tumor bearing mice, and its mechanism may be related to immune regulation and anti apoptosis.