【Abstract】 Objective To explore the effect of curcumin on PC-3 cell growth in vitro. MethodsPC-3 cells were treated with different concentration of curcumin to observe the changes on cell proliferation，cell apoptosis and detect the effect of curcumin on Notch1 and PI3K mRNA level in PC-3 cell. ResultsCell proliferation were reduced by curcumin which could also enhance the cell apoptosis by increasing Caspase-3 and Caspase-9 activity with the dose dependent manner (P<0.05，n=6）. Higher Notch1，PI3K mRNA level and protein expression were obtained in PC3 cell which could be reduced by curcumin application with the dose dependent manner (P<0.05，n=6）. ConclusionEffect of curcumin regulate PC-3 cell growth negatively in vitro.