Objective To explore the clinical effect of comprehensive treatment of traditional Chinese medicine in the treatment of vulvovaginitis in girls before puberty. Methods 98 prepubescent girls with vulvovaginitis admitted to the Department of Pediatrics，Southern District，Guang′anmen Hospital，China Academy of Chinese Medical Sciences and Pediatric Gynecology Clinic，Beijing Children′s Hospital，Capital Medical University from May 1，2020 to December 31，2020，were randomly divided into the observation group at a ratio of 2:1 (Comparing the clinical symptom scores and the disease recovery rate and recurrence rate of the two groups with the control group (oral amoxicillin and clavulanate potassium). Results There was no significant difference in symptom scores between the two groups before treatment (P>0.05). After treatment，the scores of the two groups were significantly lower than before treatment，and the clinical symptoms were significantly improved (P<0.001). The observation group and the control group had clinical symptoms after treatment. There was no statistically significant difference in score comparison (P>0.05). Comparing the cure rate (81.82%) and recurrence rate (16.67%) of the observation group with the cure rate (87.50%) and recurrence rate (18.75%) of the control group，there were no statistically significant differences (P>0.05). Conclusion The clinical efficacy of traditional Chinese medicine on vulvovaginitis in prepubertal children is not inferior to that of antibiotics，and it can be popularized for prepubertal vulvovaginitis children without specific vaginitis such as vaginal foreign bodies and pinworms.