Objective To construct lentivirus vector that interferes with FLOT2 expression， and explore the effect of inhibiting FLOT2 expression on proliferation， apoptosis， invasion and migration of colon cancer cells.Methods The expression of FLOT2 in colorectal cancer tissue and LoVo， SW480 and HT29 cells were detected by qRT-PCR， the expression of FLOT2 protein was detected by Western blotting. The expression of FLOT2 was detected by qRT-PCR and Western blotting after the lentivirus vector of FLOT2 siRNA was transfected into LoVo cells and SW480 cells. MTT assay， flow cytometry， Transwell chamber and Western blotting were used to detect the cell proliferation， apoptosis rate， invasion and migration ability and the expression of PCNA， cleaved caspase 3， E-cadherin and vimentin.Results The expression of FLOT2 mRNA and protein in three colon cancer cells was significantly higher than that in NCM460 cells (P<0.05).After FLOT2 siRNA lentiviral vector were transfected into LoVo cells and SW480 cells， the expression of FLOT2 mRNA and protein were significantly reduced， and the ability of cell proliferation， invasion and migration were significantly reduced， apoptosis was promoted， the expression of PCNA and Vimentin was down regulated， and cleaved Caspase3 and E-cadherin were up regulated (P<0.05).Conclusion Inhibition of FLOT2 expression can significantly reduce the proliferation， invasion and migration of colon cancer cells， and promote apoptosis. The mechanism may be related to the regulation of PCNA， cleaved caspase 3， E-cadherin and vimentin expression.