Objective To investigate the effect of glioma cell exosomes on angiogenesis and its mechanism.Methods Differential centrifugation was used to extract exosomes secreted by human glioma cells U373 and U87 and human glial cells HEB cells. The exosomes were observed by transmission electron microscopy， and the exosomes were identified by Western blot.0.2μg/μl exosomes and equal volume of PBS were incubated with HUVECs， CCK-8 method was used to detect cell proliferation ability， Transwell test was used to detect cell migration ability， tubule formation experiment was used to detect angiogenesis in vitro， flow cytometry was used to detect apoptosis， Western The expression of apoptotic proteins Bax， Caspase3， Caspase9， the expression of VEGF， VEGFR2 and the phosphorylation level of AKT were detected by blot.Results The exosomes extracted accorded with its characteristics. Compared with the PBS group， HUVECs had no significant changes in proliferation， migration， tubule formation， and apoptosis after co-incubation with HEB-exo； compared with U373-exo after incubation with U87-exo， HUVECs proliferation， migration， and tubule formation ability were significantly increased P<0.005)； apoptosis levels were significantly reduced (P<0.001)， and Bax， Caspase3， and Caspase9 expressions were significantly decreased (P<0.00). The expression of VEGF， VEGFR2 and the phosphorylation level of AKT were significantly increased (P<0.001).Conclusion Exosomes of glioma can activate VEGF/AKT signaling pathway and promote angiogenesis.