Objective To investigate the expression of miRNA-98 in hypopharyngeal carcinoma Fadu cells and the mechanism of its regulation on metastasis and epithelial mesenchymal transformation.Methods Real-time quantitative PCR was used to detect the mRNA expression of miR-98 and EZH2 in 35 hypopharyngeal carcinoma tissues and 28 adjacent normal tissues. The expression of miR-98 was down-regulated by transfection technique. The proliferation， invasion and epithelial mesenchymal transformation ability of Fadu cells were detected by CCK-8 assay， Transwell assay and Western blot assay， respectively. The downstream regulatory targets of miR-98 were analyzed by TargetScan， and the relationship between miR-98 and EZH2 was further detected by dual luciferase reporter assay. The expression of EZH2 was down-regulated by transfection technique， and the effects of down-regulation of EZH2 on proliferation， invasion and EMT of Fadu cells were analyzed. At the same time， the expression of miR-98 and EZH2 were up-regulated， and the effects of over-expression of miR-98 on Fadu cell metastasis and EMT through EZH2 were detected. After the down-regulation or overexpression of EZH2， the protein expressions of JAK， p-JAK， STAT3 and p-STAT3 in FADU cells were detected by Western blot. Fadu cells were treated with the inhibitor AG490， and the expression of EZH2 was up-regulated by transfection technology to detect the proliferation， invasion and EMT ability of Fadu cells. Results Compared with adjacent normal tissues， miR-98 expression was significantly reduced in hypopharyngeal carcinoma tissues （P<0.01）， the expression of EZH2 was significantly increased （P<0.01）.Downregulation of miR-98 promoted proliferation， invasion and EMT of Fadu cells. miR-98 targeted EZH2 and had a negative regulatory relationship with EZH2.The up-regulation of miR-98 inhibited the proliferation， invasion and EMT of Fadu cells by EZH2.Downregulation of EZH2 inhibited Fadu cell metastasis and EMT. The up-regulation of EZH2 activated the JAK/STAT3 pathway in Fadu cells， and AG490 can significantly inhibit the up-regulation of EZH2 overexpression on proliferation， invasion and EMT of Fadu cells. Conclusion miRNA-98 targeting EZH2 activates the JAK/STAT3 pathway to regulate hypopharyngeal cancer cell metastasis and epithelial-mesenchymal transformation.