Objective To explore the clinical efficacy and safety of dendritic cell (DC)-cytokine induced killer cell (CIK) immunotherapy combined with chemotherapy for advanced colorectal cancer (CRC). Methods From June 2013 to January 2016, 45 patients with advanced colorectal cancer who receiving DC-CIK immunotherapy combined with CapeOX chemotherapy were collected as the observation group in the Donggang District of The First Hospital of Lanzhou University. In addition, 45 patients with advanced colorectal cancer who received only CapeOX chemotherapy during the same period were collected as the control group. The immune function status, clinical efficacy and toxic reaction of the two groups of patients were observed. Results There was no significant change in T lymphocyte subsets in the observation group before and after treatment. The proportion of CD3+,CD3+CD4+, CD3+CD8+, NK and NKT cells in peripheral blood after treatment was significantly lower than before treatment in the control group, and was significantly lower than that in the observation group after treatment (P＜0.05). There was no significant difference in the response rate (RR) between the observation group and the control group. The disease control rate(DCR) in the observation group was significantly higher than that in the control group (P＜0.05). The incidence of leukopenia, thrombocytopenia and diarrhea in the observation group was significantly lower than that in the control group (P＜0.05).The progression-free survival (PFS) time of patients in the observation group was significantly longer than in the control group (P＜0.05). There was no statistically significant difference in overall survival (OS) time between the observation group and the control group. Conclusion DC-CIK immunotherapy combined with chemotherapy can significantly prolonged the progression-free survival time of patients with advanced colorectal cancer, significantly improve the patient's immune function and the anti-tumor ability of patients, and reduce the adverse reactions of chemotherapy.