Objective To investigate whether Guhong Injection improved the clinical symptoms of patients with Hepatic stress injury induced by ischemic stroke and explore the protective effects of Guhong Injection on cerebral ischemia-induced liver damage and its mechanism. Methods This was a randomized, double blind controlled trial. Patients of cerebral ischemia-induced liver damage were randomly divided into the two groups, conventional treatment (n=40) and Guhong Injection treatment (n=40). Alanine aminotransferase(ALT), aspartate transaminase(AST), alkaline phosphatase(ALP) and serum bilirubin level(TBiL)were measured on the baseline and at day 10 and 20 following treatment, at the same time, Serum tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and mitochondrial superoxide dismutase (SOD), malondialdehyde (MDA) were measured too. Results ALT, AST, ALP, TBiL and TNF-α,IL-6, SOD, MDA in the GH group were no statistically significant difference than in the CG group on the baseline(P＞0.05).The level of ALT, AST, ALP, TBiL and TNF-α,IL-6, MDA in the GH group were lower than those in the CG groups, while the level of SOD in the GH group was higher than that in the CG group at day 10 and 20, and the differences were statistically significant(P＜0.05). Conclusion Guhong Injection seems to be an efficient method that can improve the clinical symptoms of patients with Hepatic stress injury induced by ischemic stroke. Carthamus tinctorius L. of Guhong Injection may have hepatoprotective effects and antioxidant activity in liver injury.