Objective To reveal the predictive value of metastasis-associated protein 2 (MTA2) on the prognosis of prostate cancer and its role in the process of cancer metastasis. Methods From January 2018 to June 2020, a total of 50 prostate cancer tissues and paired adjacent tissue samples of The First Affiliated Hospital of Air Force Medical University were collected for immunohistochemical analysis. The samples were divided into two groups according to the expression of MTA2 protein. The staining score ≥4 was high expression, and ＜4 was low expression. Human prostate cancer cell line (PC-3) was transfected with shMTA2 (MTA2-shRNA-pLKO.1) to silence MTA2, shNC (Luc-shRNA-pLKO.1) was used as a negative control, and untransfected cells were used as a blank control (Control). Cell viability was detected by the MTT method, and the Transwells experiment was used for in vitro migration and invasion analysis. The expression of MTA2, Eotaxin-1, CCR3, p-ERK1/2, t-ERK1/2 and MMP-3 in cells was detected by qRT-PCR or Western Blot. Results The staining score of MTA2 in prostate cancer tissue was significantly higher than that in adjacent tissues (5.16±0.87vs2.34±0.39,t=9.221, P＜0.001). There were significant differences in lymph node metastasis between the two groups. 14.29% (2/14) of patients with low MTA2 expression had lymph node metastasis, and 52.78% (19/36) of patients with high MTA2 expression had lymph node metastasis (x2=6.131, P=0.013). The survival time of patients with low and high expression of MTA2 was significantly different (x2=4.756, P=0.029). Compared with the control group, the cell viability of the shMTA2 group was reduced by 56.87% (P＜0.001), the number of cell migration was reduced by 65.80% (P＜0.001), the number of cell invasion was reduced by 56.35% (P＜0.001), the relative protein expression of Eotaxin-1, CCR3, p-ERK1/2 and MMP-3 decreased by 76.03%, 69.12%, 72.32% and 54.67% respectively (P＜0.001), while the relative protein expression of t-ERK1/2 did not change significantly (P＞0.05). Conclusion The expression level of MTA2 in prostate cancer tissue is elevated and is related to the prognosis of the patient, silencing MTA2 can reduce the migration and invasion of prostate cancer cells, and inhibit the Eotaxin-CCR3-ERK1/2-MMP-3 axis.