Objective To investigate the effect of Naringin on the osteogenic differentiation, proliferation and migration and OPG/RANKL gene expression of rabbit bone marrow stromal cells. Methods 2-3 months old New Zealand white rabbits were used to extract rabbit bone marrow stromal cells. The cells were treated with different concentrations (0-1000μM) of naringin for 48 hours, and the cell viability was measured with CCK-8 kit. The cells were treated with naringin (0μM, 10μM, 25μM and 50μM) for 48 hours, alkaline phosphatase (ALP) activity measurement kit and Alizarin Red S (ARS) staining were used to determine the osteogenic differentiation ability of the cells. Cell scratch test and crystal violet staining were used to detect cell migration ability. Real-time quantitative-polymerase chain reaction (RT-PCR) method measures the levels of OPG, Runx2, OCN and RANKL mRNA in cells. Western blotting was used to measure OPG, Runx2, OCN and RANKL protein levels in cells. Results Compared with the 0μM group, 10μM, 25μM and 50μM naringin had no effect on the viability of rabbit bone marrow stromal cells. The ALP activity of rabbit bone marrow stromal cells in the naringin (25μM and 50μM) group was increased and the ARS staining was enhanced. At the same time, the cell migration ability of naringin 10μM, 25μM and 50μM groups was enhanced. The mRNA and protein levels of OPG, Runx2 and OCN in the naringin treatment group were significantly up regulated, but the RANKL mRNA and protein levels in the naringin treatment group were significantly down-regulated. Conclusion Naringin can promote the osteogenic differentiation and migration of rabbit bone marrow stromal cells, and it may be related to the regulation of OPG/RANKL gene expression.